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The second reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as items (Fig. 9) - UV/Vis. As drawn in Fig - https://www.cybo.com/US-biz/on-line-instrument-systems-olis-inc. 9, the reaction might also include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As displayed in Fig. 9, this might be due to the fact that the H4B radical function as an electron sink in a later point in the reaction, so as to permit NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.

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